Fetal Chromosome Analysis

Fetal chromosome analysis using amniocytes cultured from a sample of amniotic fluid are obtained from the patient’s pregnancy. Amniotic Fluid Chromosome Analysis (AFCA) will rule out a chromosomal abnormality with greater than 99 percent accuracy.

Indications for Testing:

  • Abnormal prenatal screening result
  • Advanced maternal age
  • Family history of a chromosome abnormality
  • Abnormal ultrasound

Aneuvysion can be added to AFCA for preliminary results (typically available in 48 to 72 hours from the time the sample reached the lab) on the number of chromosome 13, 18, 21, X, and Y present in the fetus.

Amniotic Fluid AFP Analysis (AFAFP) will also be performed on all amniotic fluid samples received by the lab from pregnancies in less than 24 weeks gestation. When combined with a second trimester ultrasound, AFAFP can detect greater than 99 percent of open neural tube defects. Acetycholinesterase analysis will be completed automatically in the presence of elevated AFAFP.

Sample Requirements

20 to 25 cc amniotic fluid collected in sterile Becton-Dickinson syringes. Please remove needle and recap the syringe with the original tip cover. Specimen can be forwarded to the lab in the original syringes or in sterile conical centrifuge tubes.

Sample Handling

Please call 216-983-1134 to arrange for a courier pick-up of a local specimen. Specimens being sent from outside facilities should be packaged according to standard medical safety practices. A cold pack should be placed in the container during warm summer months. The specimen should never be frozen. The specimen should be shipped overnight priority.

Turn Around Time

  • Aneuvysion – within 72 hours of sample receipt
  • Final Results – 10 to 14 days of sample receipt

Cost/CPT Codes

  • Amniotic Fluid Chromosome Analysis
    88235, 88267, 88280 = $756.52
  • Aneuvysion
    88271(x5), 88274(x2) = $632.80
  • Amniotic Fluid AFP Analysis
    82106 = $60.08
  • Acetylcholinesterase Analysis
    82013 = $125.00


Please submit a completed Prenatal and Postnatal Cytogenetics and Molecular specimens requisition for each sample.